Reporter

Part:BBa_K1997014:Design

Designed by: Xinyuan Qiu   Group: iGEM16_NUDT_CHINA   (2016-10-13)


GFP10->Zif268->GFP11->RBS->GFP1-9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 51
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 208
    Illegal AgeI site found at 292
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 380
    Illegal BsaI.rc site found at 112
    Illegal BsaI.rc site found at 443


Design Notes

This part is designed to be a positive control which contains the complete ZFN protein.

Source

This part was synthesized by IDT

References